Adoptive Immunotherapy : Methods and Protocols

An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients.

Exploiting Dendritic Cells for Active Immunotherapy of Cancer and Chronic Infection David O''Neill and Nina Bhardwaj A Mathematical Approach for Optimizing Dendritic Cell-Based Immunotherapy Gennady Bocharov, Neville J. Ford, and Burkhard Ludewig Delivery of Tumor Antigens to Dendritic Cells Using Biodegradable Microspheres Ying Waeckerle-Men, Bruno Gander, and Marcus Groettrup RNA Transfection of Dendritic Cells Frank Grünebach, Martin R. Müller, and Peter Brossart Isolation and Generation of Clinical-Grade Dendritic Cells Using the CliniMACS System John D. M. Campbell, Christoph Piechaczek, Gregor Winkels, Edith Schwamborn, Daniela Micheli, Sonja Hennemann, and Jürgen Schmitz Generation of Clinical Grade Monocyte-Derived Dendritic Cells Using the CliniMACS System Thomas Putz, Hubert Gander, Reinhold Ramoner, Claudia Zelle-Rieser, Andrea Rahm, Walter Nussbaumer, Georg Bartsch, Lorenz Höltl, and Martin Thurnher Adenoviral Transduction of Dendritic Cells Rienk Offringa, Kitty Kwappenberg, Martijn Rabelink, Delphine Rea, and Rob Hoeben Generation of Autologous Peptide- and Protein-Pulsed Dendritic Cells for Patient-Specific Immunotherapy David O''Neill and Nina Bhardwaj Phenotypical and Functional Characterization of Clinical-Grade Dendritic Cells I. Jolanda M. de Vries, Gosse J. Adema, Cornelis J. A. Punt, and Carl G. Figdor Dendritic Cells in Clinical Trials for Multiple Myeloma Volker L. Reichardt and Peter Brossart Identification of Tumor-Associated Autoantigens With SEREX Özlem Türeci, Dirk Usener, Sandra Schneider, and Ugur Sahin Application of Proteomics and Protein Analysis for Biomarker and Target Finding for Immunotherapy Petra Weingarten, Petra Lutter, Andreas Wattenberg, Martin Blueggel, Sonja Bailey, Joachim Klose, Helmut E. Meyer, and Christoph Huels Isolationand Expansion of Tumor-Reactive Cytotoxic T-Cell Clones for Adoptive Immunotherapy Helga Bernhard, Burkhard Schmidt, Dirk H. Busch, and Christian Peschel Tracking Adoptively Transferred Antigen-Specific T-Cells With Peptide/MHC Multimers Norbert Meidenbauer and Andreas Mackensen Gene Transfer of MHC-Restricted Receptors Helmut W. H. G. Kessels, Monika C. Wolkers, and Ton N. M. Schumacher Immunotherapy With CTL Restricted by Nonself MHC Liquan Gao, Anne-Marie Downs, and Hans J. Stauss Designing TCR for Cancer Immunotherapy Ralf-Holger Voss, Jürgen Kuball, Matthias Theobald Isolation and Expansion of Tumor-Specific CD4+ T-Cells by Means of Cytokine Secretion Christian Becker Methods for the Ex Vivo Characterization of Human CD8+ T Subsets Based on Gene Expression and Replicative History Analysis Nathalie Rufer, Patrick Reichenbach, and Pedro Romero Regulatory T Cells in Antitumor Therapy: Isolation and Functional Testing of CD4+CD25+ Regulatory T-Cells Helmut Jonuleit and Edgar Schmitt Monoclonal Antibody-Based Strategies in Autoimmunity and Transplantation Lucienne Chatenoud Producing Bispecific and Bifunctional Antibodies Dipankar Das and Mavanur R. Suresh Antibody-Cytokine Fusion Proteins for the Therapy of Cancer Gustavo Helguera and Manuel L. Penichet Synthesis and Biological Evaluation of a Paclitaxel Immunoconjugate Ahmad Safavy and Kevin P. Raisch Cytotoxic Tumor Targeting With scFv Antibody-Modified Liposomes Cornelia Marty and Reto A. Schwendener Intravenous Immunoglobin Treatment for Fibrosis, Atherosclerosis, and Malignant Conditions Ilan Krause and Yehuda Shoenfeld Study of T-Cell Costimulatory Blockade In Vivo at a Single Cell Level Minh Diem Vu and Xian Chang Li Stem Cell Transplantation: Graft-Mediated Antileukemia