Cell Encapsulation Technology and Therapeutics

Encapsulated cells offer enormous potential for the treatment of human disease. This work includes detailed descriptions of chemical properties of encapsulation materials, purification, biocompatibility issues and experimental protocols.

I Fundamentals of Cell Encapsulation.- 1. Encapsulation and Immoblization Techniques.- 2. Mass Transfer in Immobilized Cell Systems.- 3. Polymer Membranes for Cell Encapsulation.- 4. Biocompatible Encapsulation Materials: Fundamentals and Application.- 5. Modulation of Membrane Permeability.- 6. Biocompatibility Issues.- II Encapsulation Systems Section 1 Microencapsulation.- 7. Calcium Alginate.- 8. Immunoprotection of Islets of Langerhans by Microencapsulation in Barium Alginate Beads.- 9. Agarose.- 10. Poly (ethylene glycol).- 11. Long-Term Survival of Poly-L-Lysine-Alginate Microencapsulated Islet Xenografts in Spontaneously Diabetic NOD Mice.- 12. Alginate/Poly-L-Ornithine Microcapsules for Pancreatic Islet Cell ImmunoprotectionGiuseppe.- 13. Chitosan.- 14. Polyacrylates.- Section 2 Macroencapsulation.- 15. Diffusion Chambers.- 16. Vascular Devices.- 17. Macrocapsules Based on Ultrafiltration and Diffusion.- III Applications of Encapsulated Cell Systems Section 1 Primary Cells.- 18. Artificial Pancreas.- 19. Microencapsulated Islets in Type I Diabetics: Clinical Experience.- 20. Transplantation of Microencapsulated Parathyroid Tissue: Clinical Background, Methods, and Current Status of Research.- 21. Bioartificial Livers.- 22. Renal Replacement Devices: The Development of the Bioartificial Kidney.- 23. Encapsulation of Mammalian Embryos.- 24. Encapsulated Plant Cells: Techniques and Applications.- Section 2 Genetically Engineered Cells.- 25. Implantable Microcapsules for Gene Therapy for Hemophilia.- 26. Microencapsulation: A Novel Gene Therapy for Lysosomal Storage Diseases.- 27. Growth Hormone Gene Therapy Using Encapsulated Myoblasts.- 28. Transplantation of Encapsulated Cells into the Central Nervous System.- 29. Removal of Urea in Uremia and Ammonia in Liver Failure with Emphasis on the Use of Artificial Cells for Encapsulation of Genetically Engineered Cells.- Section 3 Bioreactor Culture.- 30. Principles of Bioreactor Design for Encapsulated Cells.- 31. Culture of Anchorage-Dependent Cells.