Cell-Free Gene Expression : Methods and Protocols
2022 ed.
Book Details
Format
Paperback / Softback
Book Series
Methods in Molecular Biology
ISBN-10
1071620002
ISBN-13
9781071620007
Edition
2022 ed.
Publisher
Springer-Verlag New York Inc.
Imprint
Springer-Verlag New York Inc.
Country of Manufacture
GB
Country of Publication
GB
Publication Date
Jan 7th, 2023
Print length
435 Pages
Product Classification:
Scientific equipment, experiments & techniquesGenetics (non-medical)BiochemistryBiotechnology
Ksh 25,200.00
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This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits.
This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.
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