Chemosensitivity : Volume I: In Vitro Assays

A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors.

Part I. Overview An Overview of Chemosensitivity Testing Rosalyn D. Blumenthal Part II. In Vitro Measures of Chemosensitivity Clonogenic Cell Survival Assay Anupama Munshi, Marvette Hobbs, and Raymond E. Meyn High-Sensitivity Cytotoxicity Assays for Nonadherent Cells M. Jules Mattes Sulforhodamine B Assay and Chemosensitivity Wieland Voight Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity Gertjan J. L. Kaspers Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity Hisayuki Kobayashi The MTT Assay to Evaluate Chemosensitivity Jack D. Burton Histoculture Drug Response Assay to Monitor Chemoresponse Shinji Ohie, Yasuhiro Udagawa, Daisuke Aoki, and Shiro Nozawa In Vitro Testing of Chemosensitivity in Physiological Hypoxia Rita Grigoryan, Nino Keshelava, Clarke Anderson, and C. Patrick Reynolds Chemosensitivity Testing Using Microplate Adenosine Triphosphate-Based Luminescence Measurements Christian M. Kurbacher and Ian A. Cree High-Throughput Technology: Green Fluorescent Protein to Monitor Cell Death Marylène Fortin, Ann-Muriel Steff, and Patrice Hugo DIMSCAN: A Microcomputer Fluorescence-Based Cytotoxicity Assay for Preclinical Testing of Combination Chemotherapy Nino Keshelava, Tomásþ Frgala, JirÐ í Krejsa, Ondrej Kalous, and C. Patrick Reynolds The ChemoFx® Assay: An Ex Vivo Cell Culture Assay for Predicting Anticancer Drug Responses Robert L. Ochs, Dennis Burholt, and Paul Kornblith Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models C. Patrick Reynolds and Barry J. Maurer Image Analysis Using the Fluochromasia Assay to Quantify Tumor Drug Sensitivity John F. Gibbs, Youcef M. Rustum, and Harry K. Slocum ImmunohistochemicalDetection of Ornithine Decarboxylase as a Measure of Chemosensitivity Uriel Bachrach Immunohistochemistry of p53, Bcl-2 and Ki-67 as Predictors of Chemosensitivity Mitsuyoshi Itaya, Jiro Yoshimoto, Kuniaki Kojima, and Seiji Kawasaki Index