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CRISPR-Cas Methods - 2020 ed.

By: (Edited by) Kutubuddin A. Molla , (Edited by) M. Tofazzal Islam , (Edited by) Pankaj K. Bhowmik

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Ksh 19,800.00

Format: Paperback / Softback

ISBN-10: 1071606182

ISBN-13: 9781071606186

Edition: 2020 ed.

Series: Springer Protocols Handbooks

Publisher: Springer-Verlag New York Inc.

Imprint: Springer-Verlag New York Inc.

Country of Manufacture: GB

Country of Publication: GB

Publication Date: Jul 5th, 2020

Print length: 278 Pages

Product Classification: Genetics (non-medical)

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This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

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