CRISPR Gene Editing : Methods and Protocols
1st ed. 2019
Book Details
Format
Hardback or Cased Book
Book Series
Methods in Molecular Biology
ISBN-10
1493991698
ISBN-13
9781493991693
Edition
1st ed. 2019
Publisher
Humana Press Inc.
Imprint
Humana Press Inc.
Country of Manufacture
US
Country of Publication
GB
Publication Date
Mar 26th, 2019
Print length
362 Pages
Weight
818 grams
Dimensions
18.60 x 26.30 x 2.90 cms
Product Classification:
Medical geneticsBiotechnology
Ksh 28,800.00
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Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included.
This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
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