Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures
Book Details
Format
Paperback / Softback
ISBN-10
1780407513
ISBN-13
9781780407517
Publisher
IWA Publishing
Imprint
IWA Publishing
Country of Manufacture
GB
Country of Publication
GB
Publication Date
Jun 15th, 2015
Print length
36 Pages
Product Classification:
Water supply & treatment
Ksh 14,400.00
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Molecular techniques are emerging as a rapid and powerful approach for microorganism detection. This project reviewed the efficacy of PCR assays for Cryptosporidium, Microcystis, adenovirus and ammonia oxidising bacteria, as well as candidate techniques for DNA extraction and inhibitor removal. The literature review led to an evaluation of DNA extraction kits and reagents for PCR; finalisation of assay formats; the development of PCR controls and Standard Operating Procedures (SOP); analysis of assay robustness using real samples; and completion of an Inter-laboratory trial using the SOP’s developed. This book is co-published with Water Research Australia.
Molecular techniques are emerging as a rapid and powerful approach for microorganism detection. This project reviewed the efficacy of PCR assays for Cryptosporidium, Microcystis, adenovirus and ammonia oxidising bacteria, as well as candidate techniques for DNA extraction and inhibitor removal. The literature review led to an evaluation of DNA extraction kits and reagents for PCR; finalisation of assay formats; the development of PCR controls and Standard Operating Procedures (SOP); analysis of assay robustness using real samples; and completion of an Inter-laboratory trial using the SOPs developed.
This book is co-published with Water Research Australia.
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