Expression, Purification, and Structural Biology of Membrane Proteins

This second edition provides new and updated protocols on membrane protein biology. Chapters explore methods for cloning and expression of membrane proteins, membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins, Second Edition serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins.  Chapter 11 is available open access under a CC-BY 4.0 license via link.springer.com.