Fibrosis Research : Methods and Protocols

Leading investigators review the highlights of current fibrosis research and the experimental methodologies used uncover the mechanisms that drive it.

Part I. The Clinical Scope of Fibrosis Pulmonary Fibrosis David A. Zisman, Michael P. Keane, John A. Belperio, Robert M. Strieter, and Joseph P. Lynch, III Renal Fibrosis H. William Schnaper Transforming Growth Factor-b: A Key Mediator of Fibrosis Alain Mauviel Part II. Regulation of Extracellular Matrix Metabolism: Cultured Cells Isolation and Culture of Skin Fibroblasts Laure Rittié and Gary J. Fisher Isolation and Culture of Hepatic Stellate Cells Ralf Weiskirchen and Axel M. Gressner Isolation and Phenotypic Characterization of Lung Fibroblasts Carolyn J. Baglole, Sireesha Y. Reddy, Stephen J. Pollock, Steven E. Feldon, Patricia J. Sime, Terry J. Smith, and Richard P. Phipps Methods for Measuring Type I Collagen Synthesis In Vitro David C. Rishikof, Ping-Ping Kuang, Mangalalaxmy Subramanian, and Ronald H. Goldstein Methods for Assessing the Molecular Mechanisms Controlling Gene Regulation Richard A. Rippe and Branko Stefanovic Methods for Measuring TGF-b Using Antibodies, Cells, and Mice Vladimir Jurukovski, Branka Dabovic, Vesna Todorovic, Yan Chen, and Daniel B. Rifkin Part III. Studying Fibrosis Using Animal Models Morphological Methods for Assessment of Fibrosis Rakesh K. Kumar Methods for Measuring Hydroxyproline and Estimating In Vivo Rates of Collagen Synthesis and Degradation Robin J. McAnulty Approaches to Evaluation of Fibrogenic Pathways in Surgical Lung Biopsy Specimens Cory M. Hogaboam, Kristin J. Carpenter, Holly Evanoff, and Steven L. Kunkel Animal Models for Adult Dermal Wound Healing Mary Birch, Annette Tomlinson, and Mark W. J. Ferguson Modeling Liver Fibrosis in Rodents Christothea Constandinou, Neil Henderson, and John P. Iredale Animal Models of Pulmonary Fibrosis MehrnazGharaee-Kermani, Matthew Ullenbruch, and Sem H. Phan Animal Models of Renal Fibrosis Michael Zeisberg, Mary A. Soubasakos, and Raghu Kalluri Animal Models of Cardiac Fibrosis Yao Sun and Karl T. Weber Part IV. Genetic Approaches to Studying Fibrosis Finding Fibrosis Genes: The Lung Lauranell H. Burch and David A. Schwartz Genetic Studies to Identify Hepatic Fibrosis Genes and SNPs in Human Populations Christopher Paul Day Analysis of Microarray Experiments for Pulmonary Fibrosis Nilesh B. Davé and Naftali Kaminski DNA Microarrays and Data Mining to Study Hepatic Fibrosis Bernd Schnabl, Youkyung Hwang Choi, Curt H. Hagedorn, and Ramón Bataller Analysis of Proteins Dominantly Expressed in Hepatic Stellate Cells of Activated Phenotype: The Molecular Approach for Liver Fibrosis Norifumi Kawada Index