Immobilization of Enzymes and Cells

Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology.

Immobilization of Enzymes as the 21st Century Begins: An Already Solved Problem or Still an Exciting Challenge? Jose M. Guisan Immobilization of Enzymes: A Literature Survey Beatriz M. Brena and Franciso Batista-Viera Cross-Linked Enzyme Aggregates Roger A. Sheldon, Rob Schoevaart, and Luuk M. van Langen Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups Cesar Mateo, Olga Abian, Gloria Fernández-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan, and Roberto Fernandez-Lafuente Glutaraldehyde in Protein Immobilization: A Versatile Reagent Lorena Betancor, Fernando López-Gallego, Noelia Alonso-Morales, Gisella Dellamora, Cesar Mateo, Roberto Fernandez-Lafuente, and Jose M. Guisan Practical Protocols for Lipase Immobilization Via Sol-Gel Techniques Manfred T. Reetz Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques Mònica Campàs and Jean-Louis Marty Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers Ipsita Roy and Munishwar N. Gupta Affinity Immobilization of Tagged Enzymes Silvana Andreescu, Bogdan Bucur, and Jean-Louis Marty Bioaffinity Immobilization Ipsita Roy and Munishwar N. Gupta One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Rodrigo Torres, Fernando López-Gallego, Jose M. Guisan, and Roberto Fernandez-Lafuente Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde-Dextran Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo, Gloria Fernández-Lorente, Roberto Fernandez-Lafuente, and Jose M. Guisan Purification, Immobilization,Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports Jose M. Palomo, Gloria Fernández-Lorente, Cesar Mateo, Rosa L. Segura, Claudia Ortiz, Roberto Fernandez-Lafuente, and Jose M. Guisan Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads® Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Fernando López-Gallego, Rodrigo Torres, Manuel Fuentes, Aurelio Hidalgo, Jose M. Palomo, Lorena Betancor, Gloria Fernández-Lorente, Claudia Ortiz, Olga Abian, Jose M. Guisan, and Roberto Fernandez-Lafuente Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports Tamara Montes, Fernando López-Gallego, Manuel Fuentes, Cesar Mateo, Valeria Grazu, Lorena Betancor, Jose M. Guisan, and Roberto Fernandez-Lafuente Stabilization of New Imprint Property of Glucose Oxidase in Pure Aqueous Medium by Cross-Linked-Imprinting Approach Alankar Vaidya and Lutz Fischer Reversible Covalent Immobilization of Enzymes Via Their Thiol Groups Francisco Batista-Viera, Karen Ovsejevi, and Carmen Manta Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando López-Gallego, Lorena Betancor, Noelia Alonso-Morales, Jose M. Guisan, and Roberto Fernandez-Lafuente Immobilization of Enzymes on Magnetic Particles Martina Koneracká, Peter Kopcansk´y , Milan Timko, Chenyl Nynitapal Ramchand, Zainul M. Saiyed, Michael Trevan, and Anil de Sequeira Immobilization of Proteins on Gold Surfaces José M. Abad, Marcos Pita, and Víctor M. Fernández Immobilization of Enzymes on Electrodes Gilvanda Silva Nunes and Jean-Louis Marty Immobilization of Enzymes for Use in Organic Media Patrick