In–Situ PCR Techniques
Book Details
Format
Paperback / Softback
ISBN-10
0471159468
ISBN-13
9780471159469
Publisher
John Wiley & Sons Inc
Imprint
Wiley-Liss Inc.,U.S.
Country of Manufacture
US
Country of Publication
GB
Publication Date
Jul 18th, 1997
Print length
160 Pages
Weight
272 grams
Dimensions
23.00 x 16.00 x 1.50 cms
Product Classification:
Genetics (non-medical)DNA & GenomeNeurosciencesMolecular biologyCellular biology (cytology)
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In-situ PCR is an attractive alternative to standard and fluorescence in-situ hybridization (F.I.S.H. ) that provides greatly enhanced sensitivity. This book presents protocols - tested and validated for sensitivity, precision and reproducibility - for in-situ PCR detection and localization of RNA and DNA.
This book describes comprehensive step-by-step protocols for the delineation of genetic amplification and histological detection techniques. Each procedure has been tested and validated for its sensitivity, precision, and reproducibility, and the authors give advice on the design of primers for PCR applications and on optimizing these protocols for use with plant, insect, and prokaryotic cells.
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