Optical Imaging Techniques in Cell Biology

This reference on optical imaging techniques covers the field from the basics of the optical microscope to highly advanced techniques such as Förster resonant energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM). Using a uniform style and format, it provides an understanding of the underlying principles, so readers can learn the benefits and limitations of each technique described. Topics covered include: optical contrasting techniques, fluorescence and fluorescence microscopes, three-dimensional imaging, the confocal microscope, nonlinear microscopy, advanced fluorescence techniques, and breaking the diffraction limit. Chapters include suggestions for further reading.

Optical Imaging Techniques in Cell Biology, Second Edition covers the field of biological microscopy, from the optics of the microscope to the latest advances in imaging below the traditional resolution limit. It includes the techniques—such as labeling by immunofluorescence and fluorescent proteins—which have revolutionized cell biology. Quantitative techniques such as lifetime imaging, ratiometric measurement, and photoconversion are all covered in detail.

Expanded with a new chapter and 40 new figures, the second edition has been updated to cover the latest developments in optical imaging techniques. Explanations throughout are accurate, detailed, but as far as possible non-mathematical. This edition includes appendices with useful practical protocols, references, and suggestions for further reading. Color figures are integrated throughout.