Optical Nanoscopy and Novel Microscopy Techniques

Many new methods in light and super-resolution microscopy have been developed over the last few years. This edited volume overviews the latest advances in microscopy, complete with experimental methods and data analysis of the results. Each chapter begins with a brief description of the principles of the technique for clarity and concludes with a set of problems. Cutting-edge microscopy techniques presented include STED, PALM/STORM, and light sheet microscopy.

Microscopy is at the forefront of multidisciplinary research. It was developed by physicists, made specific by chemists, and applied by biologists and doctors to better understand how the human body works. For this very reason, the field has been revolutionized in past decades.

The objective of Optical Nanoscopy and Novel Microscopy Techniques is to choose some of those revolutionary ideas and serve a general audience from broad disciplines to achieve a fundamental understanding of these technologies and to better apply them in their daily research.

The book begins with coverage of super-resolution optical microscopy, which discusses targeted modulation such as STED and SIM or localization methods such as PALM. It then discusses novel development of fluorescent probes, such as organic small-molecule probes, fluorescent proteins, and inorganic labels such as quantum dots. Finally, it describes advanced optical microscopy, such as fluorescence lifetime imaging, fiber optic microscopy, scanning ion conductance microscopy, and the joining of optics and acoustics—photoacoustic microscopy. Following each chapter, a detailed list of references is provided. Problems at the end of each chapter are also included.