Retinal Gene Therapy : Methods and Protocols
Softcover reprint of the original 1st ed. 2018
Book Details
Format
Paperback / Softback
Book Series
Methods in Molecular Biology
ISBN-10
1493985094
ISBN-13
9781493985098
Edition
Softcover reprint of the original 1st ed. 2018
Publisher
Humana Press Inc.
Imprint
Humana Press Inc.
Country of Manufacture
GB
Country of Publication
GB
Publication Date
Sep 5th, 2018
Print length
380 Pages
Product Classification:
Medical geneticsWildlife: mammalsWildlife: mammals: general interest
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Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in cell cultures, for gene augmentation and gene editing in vivo on rodents, pigs, and monkey retinas, and on human retinal explants as well as in human clinical studies.
This detailed volume describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in cell cultures, for gene augmentation and gene editing in vivo on rodents, pigs, and monkey retinas, and on human retinal explants as well as in human clinical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Retinal Gene Therapy: Methods and Protocols provides a wide range of readers from students to research experts with vital information on ocular gene therapy vector technology, in vitro and in vivo biological assays, and clinical protocols, to promote further studies for the benefit of children and adults with inherited retinal disease.
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