RNAi and Small Regulatory RNAs in Stem Cells : Methods and Protocols
Softcover reprint of the original 1st ed. 2017
Book Details
Format
Paperback / Softback
Book Series
Methods in Molecular Biology
ISBN-10
1493983865
ISBN-13
9781493983865
Edition
Softcover reprint of the original 1st ed. 2017
Publisher
Humana Press Inc.
Imprint
Humana Press Inc.
Country of Manufacture
US
Country of Publication
GB
Publication Date
Jun 12th, 2018
Print length
311 Pages
Product Classification:
Cellular biology (cytology)
Ksh 18,000.00
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This detailed collection provides an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells. Beginning with a brief introductory section, the volume continues by exploring methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, recently developed methods for miRNA expression and functional analysis, as well as usage of CRISPR/Cas 9 to knockout an individual gene for functional studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols aims to accelerate progress in this crucial field by reducing the time required to decipher and put into practice procedures published in the literature.
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