SMC Complexes : Methods and Protocols
2019 ed.
Book Details
Format
Hardback or Cased Book
Book Series
Methods in Molecular Biology
ISBN-10
1493995197
ISBN-13
9781493995196
Edition
2019 ed.
Publisher
Humana Press Inc.
Imprint
Humana Press Inc.
Country of Manufacture
GB
Country of Publication
GB
Publication Date
May 31st, 2019
Print length
336 Pages
Product Classification:
Genetics (non-medical)Proteins
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The protocols explore numerous subjects such as depletion systems to assess SMC function, genetic manipulation of SMC proteins, chromosomal, biochemical, and microscopy-based assays of SMC activity, as well as theoretical modeling of SMC activity.
This book brings together recent methods and theoretical approaches developed to dissect the activity and function of bacterial and eukaryotic Structural Maintenance of Chromosome (SMC) proteins. The protocols explore numerous subjects such as depletion systems to assess SMC function, genetic manipulation of SMC proteins, chromosomal, biochemical, and microscopy-based assays of SMC activity, as well as theoretical modeling of SMC activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, SMC Complexes: Methods and Protocols will greatly aid researchers looking into the mechanism of action of SMC complexes on DNA.
Chapters 10 and 19 are available Open Access under a CC BY 4.0 license via link.springer.com.
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