Total Internal Reflection Fluorescence (TIRF) and Evanescence Microscopies
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This book offers a complete presentation of the physics, math, and experimental setups for both TIRF and related evanescence microscopies. It covers evanescence in both fluorescence excitation or emission. It also discusses, in detail, the theory, setups, and practical biological/biochemical applications for combinations of evanescence microscopies with other optical techniques such as polarization, photobleaching, correlation spectroscopy, scattering, image enhancement, optical force, two-photon, energy transfer, structured illumination, scanning, quenching, and single molecule detection. Physical and qualitative discussions augment the rigorous math, making the book accessible and interesting to a wide range of audiences with backgrounds from biology to chemistry to physics. The book also contains useful step-by-step guides to building, modifying, and aligning TIRF microscopy systems for specialized purposes.
Key Features:
- Suitable for both basic and advanced levels
- Offers combinations with related techniques
- Relationship of evanescent effects between excitation and emission explained
- Practical guide to the optics as well
Get Total Internal Reflection Fluorescence (TIRF) and Evanescence Microscopies by at the best price and quality guaranteed only at Werezi Africa's largest book ecommerce store. The book was published by Institute of Physics Publishing and it has pages.