Heterologous Gene Expression in E.coli : Methods and Protocols
Softcover reprint of the original 1st ed. 2017
Book Details
Format
Paperback / Softback
Book Series
Methods in Molecular Biology
ISBN-10
1493983288
ISBN-13
9781493983285
Edition
Softcover reprint of the original 1st ed. 2017
Publisher
Humana Press Inc.
Imprint
Humana Press Inc.
Country of Manufacture
US
Country of Publication
GB
Publication Date
Jul 20th, 2018
Print length
429 Pages
Product Classification:
Microbiology (non-medical)
Ksh 19,800.00
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This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E.
This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.
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