Nanoscopy and Multidimensional Optical Fluorescence Microscopy

A complete overview of the latest developments in optical microscopy that enable structure determination at the nanoscale, this timely book draws contributions from top experts in their respective fields, including those who have either led the current upsurge in research or who have laid the foundation for the development of modern microscopes and techniques. Contributors offer state-of-the-art treatment of critical topics in nanoscopy, from imaging and manipulation to spectroscopy and visualization. Interdisciplinary in scope and approach, the book combines the science and tools of biologists, physicists, and engineers.

"Alberto Diaspro has been choreographing light’s dance for over 20 years,
and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets."
— From the Foreword by Daniel Evanko, editor, Nature Methods

Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application.

The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build.

Offering a complete treatment of this emerging field, this volume:

• Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy



• Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules



• Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample



• Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy



• Reviews the basics of FRET (